RNAi-based validation of antibodies for reverse phase protein arrays
نویسندگان
چکیده
منابع مشابه
Monitoring Proteins and Protein Networks Using Reverse Phase Protein Arrays
Recent advances in high throughput, high content "omic" technologies coupled with clinical information has lead to the expectation that the complexity of the molecular information generated will lead to more robust scientific research as well as the expectation that overarching therapeutic approaches will be patient-tailored to the underlying specific molecular defects of the disease. As diseas...
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MOTIVATION Reverse phase protein arrays (RPPA) measure the relative expression levels of a protein in many samples simultaneously. A set of identically spotted arrays can be used to measure the levels of more than one protein. Protein expression within each sample on an array is estimated by borrowing strength across all the samples, but using only within array information. When comparing acros...
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Protein microarrays offer a new means by which to conduct quantitative profiling of disease-associated proteins. The knowledge gained may provide novel strategies for early detection, diagnosis and therapeutic intervention. A variety of sophisticated approaches, including gene arrays, sequencing consortiums and large-scale two-dimensional gel electrophoresis, continue to generate lists of prote...
متن کاملTechnique review Utility of reverse phase protein arrays: Applications to signalling pathways and human body arrays
Protein microarrays offer a new means by which to conduct quantitative profiling of diseaseassociated proteins. The knowledge gained may provide novel strategies for early detection, diagnosis and therapeutic intervention. A variety of sophisticated approaches, including gene arrays, sequencing consortiums and large-scale two-dimensional gel electrophoresis, continue to generate lists of protei...
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CdSe nanocrystals, also called quantum dots (Qdots) are a novel class of fluorophores, which have a diameter of a few nanometers and possess high quantum yield, tunable emission wavelength and photostability. They are an attractive alternative to conventional fluorescent dyes. Quantum dots can be silanized to be soluble in aqueous solution under biological conditions, and thus be used in bio-de...
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ژورنال
عنوان ژورنال: Proteome Science
سال: 2010
ISSN: 1477-5956
DOI: 10.1186/1477-5956-8-69